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在中部前额叶皮层内源性阿片类系统调解胺的抗抑郁药类作用
Cheng Jiang1, Ralph J DiLeone2,3, Christopher Pittenger4,5,6,7
1Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA. Cheng.jiang@yale.edu.
内源性阿片类系统,特别是介质前额叶皮层 (mPFC) 中的β-endorphin,对胺酸至关重要.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 建议内源性阿片类系统在胺的抗抑郁作用中的作用,但尚未完全理解.
- 澄清特定的大脑区域和涉及的分子通路是必不可少的.
研究的目的:
- 调查前额前内源性阿片类系统对胺类似抗抑郁药的作用的贡献.
- 阐明基他胺在中间前额叶皮层 (mPFC) 作用的分子机制.
主要方法:
- 在大鼠模型中利用了药理,行为和分子技术的组合.
- 系统性地和直接进入mPFC中使用胺和纳尔特雷克松 (阿片类受体对抗剂).
- 测量了β-endorphin水平,mu-opioid受体基因表达 (Oprm1) 和proopiomelanocortin基因表达.
主要成果:
- 系统性和mPFC内部的纳尔特雷克松阻断了胺的行为和分子效应.
- 胺增加了mPFC中的β-endorphin和Oprm1的表达.
- 胺增加了在下丘脑中的proopiomelanocortin基因表达.
- 在mPFC中中和β-endorphin消除了胺的作用.
结论:
- 在mPFC中存在β-endorphin和阿片类受体的激活对于胺的抗抑郁药类作用是必要的.
- 这些发现凸显了前额前类药物系统在调解胺治疗效果方面的关键作用.
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