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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 3, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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使用纳米-DMS-MaP确定异形特异性RNA结构.

Anne-Sophie Gribling-Burrer1, Patrick Bohn2, Redmond P Smyth3,4

  • 1Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany. anne-sophie.gribling@helmholtz-hiri.de.

Nature protocols
|February 12, 2024
PubMed
概括
此摘要是机器生成的。

纳米孔二甲基硫酸盐突变概况 (Nano-DMS-MaP) 确定特定异构体的RNA结构. 这种方法使用纳米孔测序揭示了长,类似的RNA分子中隐藏的结构差异.

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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生物化学

背景情况:

  • 了解RNA结构对于阐明其生物功能至关重要.
  • 细胞表达多种RNA异型,其序列因替代拼接和差异多化等过程而变化.
  • 现有的RNA结构确定方法经常与长且高度相似的RNA分子相斗争.

研究的目的:

  • 描述纳米孔二甲基硫酸盐突变分析 (Nano-DMS-MaP) 的开发和应用.
  • 为了在现场进行异构体特异性RNA结构的确定.
  • 解决长和高度相似的RNA分子中的结构差异.

主要方法:

  • 使用纳米孔测序进行长读RNA结构分析.
  • 使用二甲基硫酸盐突变分析 (DMS-MaP) 来进行RNA结构的化学探测.
  • 提供了一个从实验设计和执行到数据分析的协议.

主要成果:

  • 在复杂的混合物中,纳米-DMS-MaP成功地解决了单个异构体的RNA结构.
  • 该方法揭示了高度相似的RNA分子中以前隐藏的结构变异.
  • 对于经验丰富的研究人员来说,细胞内探测实验在3-4天内是可行的.

结论:

  • 纳米-DMS-MaP是一种强大的工具,用于确定异形特异性RNA结构.
  • 这种方法克服了用于分析复杂RNA结构的短读序列的局限性.
  • 该协议促进了纳米-DMS-MaP的应用,用于全面的RNA结构研究.