相关实验视频
Updated: Jul 3, 2025

10:34
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
Published on: April 23, 2017
7.0K
MlaD的结构特征揭示了其独特的联体运输机制和祖先
Angshu Dutta1, Shankar Prasad Kanaujia2
1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
The protein journal
|February 12, 2024
概括
这项研究揭示了大肠杆菌MlaD的晶体结构,这是一种膜蛋白,对细菌中的脂运输至关重要. 它提出了一种新的"非对称原体运动"机制用于脂质运输,并突出了MlaD.
科学领域:
- 结构生物学是结构生物学.
- 细菌外膜的运输方式
- 分子机制的分子机制
背景情况:
- MlaD蛋白是一种与膜相关的溶解物结合蛋白 (SBP),参与维护格兰负细菌中的脂质不对称性.
- 以前的研究提供了结构信息,但对脂运输的分子机制和MlaD的祖先缺乏清晰度.
- 了解这些方面对于破译细菌包膜生物发生和潜在的治疗点至关重要.
研究的目的:
- 阐明来自大肠杆菌 (EcMlaD) 的MlaD周等离子体区域的晶体结构.
- 调查EcMlaD.介导的脂转运的分子机制.
- 确定EcMlaD.的进化祖先和寡合行为.
主要方法:
- 采用X射线晶体学来确定EcMlaD的结构,分辨率在2.3-3.2 Å.之间.
- 进行了生物信息和结构分析,以了解蛋白质寡合化和功能域.
- 基于结构观测,提出了一种新的机制"非对称原体运动" (APM).
主要成果:
- 晶体结构显示EcMlaD是一个具有疏水中心通道的homo-hexameric环.
- 鉴定出C端螺旋域 (HD) 对于寡合状态至关重要,与N端MlaD域不同.
- 为脂运输提出了一种独特的"非对称原体运动" (APM) 机制,涉及中央通道的动态变化.
- EcMlaD表现出类似于EF/AMT类型β6桶蛋白的折叠,这表明它与典型的SBP有着独特的祖先.
结论:
- EcMlaD作为一种非正规的SBP功能,具有独特的脂运输机制.
- 这项研究为Mla系统的结构基础和分子动力学提供了重要的见解.
- 这些发现有助于理解细菌脂质稳态,并为未来关于MlaD功能和演变的研究开辟了道路.
相关概念视频
Ligand Binding Sites
12.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.8K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
Ligand-gated Ion Channels
12.4K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
12.4K
Ligand Binding and Linkage
4.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K
Membrane Transporters
11.0K
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
11.0K
Drug Absorption Mechanism: Carrier-Mediated Membrane Transport
3.8K
Certain large, lipid-insoluble drug molecules that resemble amino acids, peptides, or glucose, require specialized carrier proteins to facilitate their diffusion across cell membranes. This transport can occur through either facilitated diffusion, which does not require energy input, or active transport, which does require energy input.
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
3.8K

