肉和层之间的表型差异在发育过程中在分子层面上受到调节
Renata Erbert Contriciani1, Carla Vermeulen Carvalho Grade2, Igor Buzzatto-Leite1
1Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
BMC genomics
|February 12, 2024
概括
肉和层在早期发育过程中表现出不同的基因表达. 肉胚胎有利于细胞增殖和延迟分化,可能会促进肌肉生长,从而改善家禽的繁殖.
科学领域:
- 基因组学和转录基因组学
- 比较的发育生物学.
- 禽畜科学是一门学科.
背景情况:
- 禽类的表型变异对于养殖计划至关重要.
- 巴西肉 (TT) 和层 (CC) 线具有明显的生长和肌肉质量.
- 早期胚胎发育的差异是理解这些特征的关键.
研究的目的:
- 在TT和CC线上比较翅膀和胸部组织的全球转录组.
- 在早期发育 (2.5-3.5天) 期间识别基因表达和调节差异.
- 发现不同增长潜力的分子基础.
主要方法:
- 来自 brojler 和 layer 胚胎的翅膀和胸部组织的转录组测序.
- 差异基因表达分析以识别DEGs (差异表达的基因).
- 基因本体学 (GO) 丰富分析和监管网络建设.
主要成果:
- 在肉和层线之间观察到明显的转录基因特征.
- 牛肉中升级的DEG被丰富为蛋白酶基因,与激素反应和细胞周期有关.
- 下调的DEG与受体,配体,细胞信号和分化有关.
结论:
- 在早期发育过程中,肉和层系之间存在显著的转录基因差异.
- brojiler胚胎表现出基因表达,有利于增加细胞增殖和延迟分化.
- 这些发现为改进目标家禽特征和繁殖策略提供了见解.
相关概念视频
Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
Complementation Tests
4.9K
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
4.9K
Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
Gene Duplication and Divergence
6.1K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.1K
Determination
18.5K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
18.5K
Convergent Evolution
27.7K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
27.7K


