1,2,4-butanetriol的高效生产从玉米水解由代谢工程大肠杆菌
Ping Li1,2, Mengjiao Wang1, Haiyan Di1
1State Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Microbial cell factories
|February 12, 2024
概括
这项研究利用大肠杆菌将玉米废物转化为1,2,4-butanetriol,一种有价值的化学物质. 改造品种取得了高产量,证明了可持续生物生产的潜力.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 玉米是一种丰富的农业废物.
- 玉米水解剂含有可发酵的糖类,如糖和葡萄糖.
- 1,2,4-butanetriol是一种多功能平台化学物质,具有众多的应用.
研究的目的:
- 从玉米水解液中设计一种复合大肠杆菌菌株,以高效地生产1,2,4-butanetriol.
- 为了克服诸如催化剂抑制和增强辅因子供应等挑战,以改善1,2,4-butanetriol合成.
主要方法:
- 通过删除ptsG和pgi基因对大肠杆菌进行基因改造,以减少触媒抑制并增加NADPH可用性.
- 引入编码西洛斯脱酶,西洛诺拉克托纳酶,西洛纳酸脱酶和α-基酸脱酶的异构基因.
- 工程化大肠杆菌菌株BT-10的发酵,使用西洛斯和玉米水解剂作为基质.
主要成果:
- 经过工程改造的大肠杆菌菌株BT-10从西洛中产生了36.63g/L的1,2,4-butanetriol,其生产率为1.14g/[L·h].
- 使用玉米水解剂,该菌株的度为43.4g/L1,2,4-butanetriol,生产率为1.09g/[L·h],产量为0.9mol/mol.
- 删除ptsG和pgi成功地减轻了catabolite抑制,并增强了NADPH供应.
结论:
- 开发的复合大肠杆菌菌株BT-10对于将玉米水解盐转化为1,2,4-butanetriol有效.
- 这种工程菌株对1,2,4-butanetriol的经济高效和可持续的商业生产有显著的前景.
- 采用的代谢工程策略可以应用于使用纤维素生物质的其他生物生产过程.
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