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在中国广泛耐药性高病毒性 Pseudomonas aeruginosa 隔离物的分子表征

Jun Li^1,2, Mengli Tang¹, Zhaojun Liu¹

¹Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.

Annals of clinical microbiology and antimicrobials

|February 12, 2024

概括

具有广泛的耐药性高病毒性Pseudomonas aeruginosa (XDR-hvPA) 异常常见,特别是在老年人中. 这项研究强调需要加强对老年患者XDR-hvPA的监测,以防止其传播.

科学领域:

临床微生物学和传染病.
基因组学和分子流行病学.
抗微生物耐药性抗微生物耐药性

背景情况:

广泛耐药的Pseudomonas aeruginosa (XDR-PA) 存在重大临床挑战.
关于广泛耐药性高病毒性P. aeruginosa (XDR-hvPA) 的数据有限,需要对其特征和传播进行进一步调查.

研究的目的:

调查XDR-hvPA的流行病学特征.
为防止XDR-hvPA的传播提供基础.

主要方法:

使用MALDI-TOF MS.收集和识别临床XDR-PA分离物 (2018-2023年) 的方法.
通过微稀释进行抗生素敏感性测试,以确定MIC.
使用Galleria mellonella模型和全基因组测序进行分子特征 (耐药性/病毒性基因,同质性) 的病毒性评估.

主要成果:

在77个XDR-PA菌株中,有47个被确定为XDR-hvPA,在60岁以上的患者中检测率更高.
在XDR-hvPA菌株中,碳烯酶基因 (例如,bla_GES-1,bla_VIM-2) 和关键毒性因子 (exoU,exoT,exoY,exoS) 是普遍存在的.
血清型O11,O7和O4,以及序列类型ST235,ST1158和ST1800是最常见的;携带blaGES的ST235XDR-hvPA菌株对塞夫塔齐/阿维巴克坦表现出敏感性.

结论:

检测到XDR-hvPA的比例很高,特别是在老年患者 (>60岁).
观察到XDR-hvPA的克隆传播,特别是携带bla_GES的ST235菌株.
在住院的老年患者中加强对XDR-hvPA的监测对于制其传播至关重要.

关键词:

临床特征临床特征药物耐药机制药物耐药机制广泛的耐药性广泛的耐药性分子流行病学分子流行病学伪omonas aeruginosa 这种类型的病毒.病毒性病毒性

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