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调节过度表达的 lncRNA ATP2B1-AS1 对肺腺癌进展的作用
Shiyi Chen1, Chao Huang1, E Jin2
1Department of Medical Oncology Ward 1, The 4th People's Hospital of Shenyang, No. 20, Huanghe South Street, Huanggu District, Liaoning, 110000, China.
Journal of cardiothoracic surgery
|February 12, 2024
概括
长非编码RNA ATP2B1-AS1 (ATP2B1-AS1) 在肺腺癌 (LUAD) 中是下调的. 它的高表达海绵miR-141-3p,缓解LUAD的进展,并表明预后价值.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 长非编码RNA ATP2B1-AS1 (ATP2B1-AS1) 与各种疾病有关.
- 它在肺腺癌 (LUAD) 中的特定作用仍然在很大程度上未被描述.
研究的目的:
- 为了研究LUAD中ATP2B1-AS1的表达模式.
- 确定ATP2B1-AS1对LUAD患者存活率和预后的影响.
主要方法:
- 实时定量聚合酶连锁反应 (RT-qPCR) 用于测量在LUAD组织和细胞中的ATP2B1-AS1和miR-141-3p表达.
- 细胞计数套件-8 (CCK-8) 和Transwell测试评估ATP2B1-AS1对LUAD细胞增殖,迁移和入侵的功能影响.
- 卡普兰-梅尔曲线和多变量考克斯回归分析,以评估ATP2B1-AS1.1.的预后意义.
主要成果:
- 与正常对照组相比,ATP2B1-AS1表达在LUAD组织和细胞中显著下调.
- 在A549 LUAD细胞中,ATP2B1-AS1的过度表达导致了增殖,迁移和入侵的减少.
- 高ATP2B1-AS1表达,作为miR-141-3p的海绵,与LUAD患者的良好预后有关.
结论:
- ATP2B1-AS1,通过海绵化miR-141-3p,有效地缓解了LUAD的进展.
- ATP2B1-AS1显示出作为肺腺癌的一个有价值的预后生物标志物的潜力.
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