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在光显微镜中计算皮尔森相关系数的像素选择标准上

Sergio G Lopez¹, Sebastian Samwald¹, Sally Jones¹

¹Cell and Developmental Biology, The John Innes Centre, Norwich Research Park, Norwich, UK.

Journal of microscopy

|February 13, 2024

概括

了解皮尔森的理解

科学领域:

细胞生物学细胞生物学
显微镜技术显微镜技术
生物物理学的生物物理.

背景情况:

定位显微镜对于评估生物分子共发生至关重要.
皮尔森相关系数 (PCC) 是一个常见的度量,但它的解释因像素选择而有所不同.

研究的目的:

评估不同像素选择标准 (PCC_ALL,PCC_OR,PCC_AND) 对色彩分析的影响.
为了指导PCC变体的适当应用,以便准确的生物学解释.

主要方法:

使用三种不同的像素选择策略对皮尔森相关系数 (PCC) 的分析:PCC_ALL (所有像素),PCC_OR (在任何道中的像素都超过值),PCC_AND (在两个道中的像素都超过值).

主要成果:

PCC_ALL对前景与背景比率敏感,引入非特定的影响.
PCC_AND有效地检测到低水平的同居化,但不太适合用于空间共发生的评估.
PCC_OR提供了信号比例性和空间巧合之间的平衡,尽管它有时可能夸大了同时出现的缺失.

结论:

每个PCC变体 (PCC_AND,PCC_OR,PCC_ALL) 都具有独特的优势和局限性.
PCC_AND最好用于检测最小的同居化.
PCC_OR提供了一个平衡的衡量标准,而PCC_ALL则有误解的风险,但通过避免细分来简化分析.

关键词:

皮尔森的相关系数系数生物分子相互作用定色化是一种定色化.光是一种光.显微镜显微镜是指使用显微镜.

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