通过将结合到三个调节部位来激活吸收调节器
Yunchan Choi1, Junseock Koh2, Sun-Shin Cha3
1Laboratory of Molecular Microbiology, School of Biological Sciences, College of Natural Science, Seoul National University, Seoul 08826, Republic of Korea.
Nucleic acids research
|February 13, 2024
概括
研究人员在细菌金属调节器Zur中发现了第四个结合部位,这对其调节功能至关重要. 这一发现增强了对稳态和金属蛋白机制的理解.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 苏尔是苏尔家族的金属调节剂,控制细菌的稳态.
- 斑点菌 (Streptomyces coelicolor Zur (ScZur)) 调节的吸收 (znuA) 和出口 (zitB) 的基因.
- 之前的研究确定了ScZur中的三个结合位,影响其结构和DNA结合.
研究的目的:
- 为了调查ScZur潜在的第四结合点的作用.
- 阐明ScZur在平衡中的调节功能机制.
主要方法:
- 生物化学测定 生物化学测定
- 基因分析 基因分析
- 异热定位热量计 异热定位热量计
- 热力学建模 热力学建模
主要成果:
- 确定了涉及histidine 36 (H36) 的第四个结合位点,并且被证明对调节功能至关重要.
- H36对于六米与zitB zurbox结合并激活zitB基因至关重要.
- 通过所有三个调节位的和,对znuA和zitB zurboxes的DNA结合亲和力显著增强.
- 一个热力学模型揭示了和DNA结合之间的强合,导致双相zitB基因激活.
结论:
- H36残留物和第四个结合点对于ScZur的监管活动至关重要.
- 祖尔的DNA结合亲和力是由结合位和度调节的.
- 这些发现表明,金属蛋白通过多个金属结合点来扩大其反应谱的机制.
相关概念视频
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
Eukaryotic Transcription Activators
11.0K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
11.0K
Cooperative Binding of Transcription Regulators
6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Prokaryotic Transcriptional Activators and Repressors
21.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.1K


