热冲击耐受性,高温生长和Salmonella Typhimurium中的毒性表达之间的进化权衡
Daniel Berdejo1,2, Julien Mortier1, Alexander Cambré1
1Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium.
mBio
|February 13, 2024
概括
沙门氏菌Typhimurium通过DNAJ基因突变获得耐热性,但这减少了生长和毒性. 这种权衡强调了应激适应和病原体适应性之间的复杂关系.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 食品安全 食品安全
背景情况:
- 食物传播的病原体,如沙门氏菌Typhimurium具有应激反应和毒性机制.
- 适应环境压力可能会影响其他环境中的病原体适应性,例如宿主殖民.
研究的目的:
- 为了研究热冲击耐药性和沙门氏菌Typhimurium中毒性之间的进化权衡.
- 了解获得耐热性的遗传基础及其对细菌适应性和致病性的影响.
主要方法:
- 沙门氏菌Typhimurium多次暴露在热冲击条件下.
- 基因分析以确定赋予耐热性的突变.
- 在不同温度下评估增长率.
- 在小鼠模型中进行病毒性测试.
主要成果:
- 沙门氏菌Typhimurium通过dnaJ基因的功能丧失突变可复制地获得了耐热冲击的增加.
- 具有增强耐热性的突变体在生理温度 (37°C及以上) 上表现出减弱的生长.
- DnaJ功能的丧失导致病毒性调节器HildD的下调,从而减少小鼠的病毒性.
结论:
- 沙门氏菌Typhimurium的热冲击适应性涉及压力抵抗和生长/毒性之间的权衡.
- 在dnaJ中的突变提供了快速耐热的机制,但在其他环境中损害了适应性.
- 该研究揭示了热应激适应和减少毒性之间的联系,通过HildD调节进行介导.
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