相关实验视频

Updated: Jul 3, 2025

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

双离心是一种新且高效的方法,用于制备脂盘

Sajid Ali¹, Jonas K Koehler², Luís Silva¹

¹Department of Chemistry - Ångström Laboratory, Uppsala University, 75237 Uppsala, Sweden.

International journal of pharmaceutics

|February 13, 2024

概括

双离心 (DC) 提供了一种新的,无溶剂的方法,用于制造小,均的聚乙烯糖醇 (PEG) 稳定脂盘. 这种技术有效地封装了黄素和多克索鲁比辛等模型药物,显示了多克索鲁比辛的高封装效率.

科学领域:

纳米技术纳米技术
材料科学材料科学材料科学
制药科学制药科学

背景情况:

聚乙烯糖醇 (PEG) 稳定型脂盘是药物输送的有希望的纳米载体.
脂质组成和制备方法极大地影响脂质盘的特性,如尺寸和药物负载.
需要新的生产技术来优化脂盘配方.

研究的目的:

研究双离心 (DC) 作为生产PEG稳定脂盘的新方法.
评估DC在封装模型药物库尔库和多克索鲁比中的有效性.
为了描述DC生产的脂质磁盘的尺寸,均性和药物负载.

主要方法:

使用双离心 (DC) 进行脂盘制备,无需有机溶剂预混合.
研究了无胆固醇和含胆固醇的脂质配方.
评估了脂盘的水力动力直径和均性.
对封装药物的量化封装效率和药物与脂质的比率.

主要成果:

电流产生了小 (20-30纳米的水力动力直径) 和均的PEG稳定脂盘.
这种技术对不含胆固醇和含有胆固醇的脂盘有效.
库尔库和多克索鲁比成功地使用DC进行了封装.
多克索鲁比辛配方实现了84%的封装效率和0.13的药物与脂质比率.

结论:

双离心法是一种快速,简单,无溶剂的方法,用于生产均的PEG稳定脂盘.
DC是一种多功能技术,适用于各种脂质组合和药物封装.
基于直流的脂盘配方显示出有效的药物输送应用的潜力.

关键词:

化电磁波是一种冷电磁波.库尔库明是一种黄素.德克索鲁比辛是一种多克索鲁比辛.药物输送是药物输送的过程.通过双重离心法进行离心.脂质盘可以使用.

更多相关视频

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

Published on: April 19, 2019

相关概念视频

Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...