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三糖6酸盐合成酶基因rdtps1有助于Rhyzopertha dominica的热适应

Dingrong Xue¹, Yan Yang^1,2, Liwei Fang³

¹National Engineering Research Center of Grain Storage and Logistics, Academy of National Food and Strategic Reserves Administration, No. 11 Baiwanzhuang Street, Xicheng District, 100037, Beijing, China.

|February 13, 2024

概括

三糖在较小的谷物钻探者 (Rhyzopertha dominica) 的耐热性中起着关键作用. 这项研究确定了三糖作为管理这种重要的储存谷物害虫的潜在目标.

科学领域:

储存产品昆虫学储存产品昆虫学
昆虫分子生物学昆虫分子生物学
害虫管理害虫管理害虫管理

背景情况:

较小的谷物挖掘机 (Rhyzopertha dominica) 是储存谷物的全球主要害虫,造成重大经济损失和食品安全问题.
这种昆虫表现出明显的适应温度波动,特别是热量.
了解其耐热性分子机制对于有效的害虫控制至关重要.

研究的目的:

在各种温度条件下研究R. dominica的基因表达差异.
阐明了昆虫适应热量的基础上的分子机制.
确定害虫管理策略的潜在目标.

主要方法:

在暴露于寒冷 (5°C,15°C),环境 (27°C) 和热 (40°C) 压力下的R. dominica的转录组分析 (RNA-seq).
不同基因表达分析和基因和基因组京都百科全书 (KEGG) 路径丰富分析.
RNA干扰 (RNAi) 试验验证候选基因的功能,包括与三糖相关的基因.

主要成果:

在温度处理中观察到基因表达的显著差异,与对照组相比,在40°C时有875个差异表达基因 (DEGs).
凯格分析强调了与脂肪酸代谢,AMPK信号传递和寿命相关的途径的丰富.
参与热冲击蛋白,脂肪酸合成和三糖代谢的基因被确定为温度调节的关键. 对与三糖相关的基因 (例如,rdtps1) 的RNAi沉默降低了三糖水平和在高温下生存.

结论:

醇是R. dominica.对高温耐受性的一个关键因素.
这项研究阐明了这种害虫耐热性分子机制.
这些发现为开发针对R. dominica.的新型害虫管理策略提供了潜在的分子标.

关键词:

它们是RNAiRNAi.里索珀塔 (Rhyzopertha dominica) 是一个多米尼加种.温度温度是指温度.对转录组进行分析.三糖6酸盐合成酶的使用方法

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