对型菌根菌检测法进行评估,以识别菌根菌
Bruno Luukinen1,2,3, Jenni Antikainen1, Janne Aittoniemi2
1Department of Clinical Microbiology, HUSLAB, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Journal of clinical microbiology
|February 13, 2024
概括
新型型菌细菌试验为结核病和非结核菌 (NTM) 提供了方便的识别. 然而,其临床性能显示某些NTM物种的识别率不足于最佳,需要仔细评估才能广泛使用.
科学领域:
- 临床微生物学 临床微生物学
- 分子诊断学 分子诊断学
- 传染病诊断 传染病诊断 传染病诊断
背景情况:
- 准确识别Mycobacterium结核菌和非结核菌 (NTM) 对于疾病管理至关重要.
- 已建立的方法,如基因型Mycobacterium CM测定,用于真菌细菌的识别.
- 新型诊断试验旨在提高速度,准确性和工作流程效率.
研究的目的:
- 评估新型型真菌细菌试验的临床性能.
- 评估其作为基因型Mycobacterium CM试验的替代品的可行性,用于初始的菌根细菌识别.
- 为了比较FluoroType测试的识别准确度和工作流程与现有的GenoType CM测试.
主要方法:
- 使用191种临床菌根培养物进行型菌根检测的临床评估.
- 对积极百分比协议 (PPA) 和特定物种的协议进行分析.
- 在注册数据的形分析以估计识别的充分性.
- 测试工作流程和结果解释与基因型CM测试的比较.
主要成果:
- 型试验显示,对158种已识别的培养物,总体阳性百分比一致率为87.8%.
- 在29个物种中,23个物种的表现完全一致,但Mycobacterium gordonae,Mycobacterium interjectum和Mycobacterium intracellulare的表现低于最佳.
- 在分析表明,与基因型CM (14.2%) 相比,型的不充分识别率 (18.8%) 较高.
- 型试验在工作流程和结果解释方面提供了显著改善的便利性.
结论:
- 型Mycobacteria测定是识别mycobacteria的一个方便的工具,但对某些NTM物种表现不佳.
- 它作为主要识别方法的可行性需要根据当地NTM物种分布进行批判性评估.
- 虽然提供了工作流的优势,但在具有普遍挑战性NTM物种的环境中,该试验的临床实用性可能是有限的.
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