麦格纳波特大米菌效应剂AvrPik-D针对转录因子WG7抑制大米免疫力
Tao Yang1, Linlin Song1, Jinxian Hu1
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 35002, China.
Rice (New York, N.Y.)
|February 13, 2024
概括
米爆真菌Magnaporthe oryzae使用效应剂AvrPik-D通过激活转录因子WG7来促进感染. 删除WG7可以增强大米对这种破坏性植物病的抵抗力.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 遗传学 是一个遗传学.
背景情况:
- 由Magnaporthe oryzae引起的水爆发严重影响水产量和水质量.
- 在成功感染的情况下,M. oryzae使用效应蛋白来破坏宿主免疫力.
- 效应因子AvrPik-D与宿主因子之间的相互作用,以及它在疾病严重性中的作用,仍然在很大程度上未被描述.
研究的目的:
- 阐明通过M. oryzae效应剂AvrPik-D增强爆病的分子机制.
- 为了研究AvrPik-D与宿主核蛋白之间的相互作用.
- 确定转录因子WG7在大米免疫中的作用及其通过AvrPik-D.调节的作用.
主要方法:
- 酵母两种混合分析以确定AvrPik-D.的相互作用伙伴.
- 对AvrPik-D和WG7.7的核定位研究.
- 为WG7.7进行转录活动测定.
- 生成和分析WG7淘汰赛 (KO) 和过度表达 (OE) 转基因大米线.
- 在转基因系中评估M. oryzae感染,活性氧物种 (ROS) 爆发和植物激素水平 (酸 - SA,酸 - JA).
主要成果:
- M. oryzae 效应剂 AvrPik-D 与米核中的指型转录因子 WG7 相互作用,促进其转录活性.
- 在大米中WG7淘汰赛显著提高了对M. oryzae的抵抗力,并在基治疗后增加了ROS爆发.
- WG7 KO植物呈现高SA水平和降低JA水平,这表明WG7通过酸途径负面调节抗性.
- 在大米系中过度表达WG7会降低对M. oryzae的抵抗力.
- WG7对于Pikh介导的抗米爆发抗药性来说并不重要.
结论:
- M. oryzae 效应剂 AvrPik-D 准并增强宿主转录因子 WG7.7 的转录活性.
- WG7在抑制大米天生的免疫力方面发挥着至关重要的作用,从而促进M. oryzae感染.
- 针对WG7代表了增强大米抗爆病的潜在策略.
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