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原生殖细胞的净化和转染方法

Luiza Chojnacka-Puchta1, Dorota Sawicka2

  • 1Department of Chemical, Biological and Aerosol Hazards, Central Institute for Labour Protection-National Research Institute, Warsaw, Poland. lucho@ciop.pl.

Methods in molecular biology (Clifton, N.J.)
|February 14, 2024
PubMed
概括

本研究介绍了一种Percoll梯度方法,用于从小胚胎中分离可活的原始生殖细胞 (PGC). 这种技术使这些重要的细胞能够有效地培养和基因修饰以供后代使用.

关键词:
的原始生殖细胞电穿孔是一种电子穿孔.免疫细胞染色检测 免疫细胞染色检测脂质受精的方法 脂质受精珀科尔密度梯度的梯度是什么

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