通过SOX17和PRDM1表达,从多能干细胞诱导人体原始生殖细胞样细胞
Naoko Irie1,2, Toshihiro Kobayashi3,4, M Azim Surani5,6
1Wellcome Trust/Cancer Research U.K. Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK. irienaoko@ciea.or.jp.
Methods in molecular biology (Clifton, N.J.)
|February 14, 2024
概括
研究人员开发了一种新方法,从干细胞中制造人类原始生殖细胞样细胞 (PGCLC). 这种技术使用特定的基因表达,SOX17和PRDM1,为研究生殖细胞发育和生殖医学提供了至关重要的工具.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 生殖生物学 生殖生物学
背景情况:
- 人类原始生殖细胞 (PGC) 的发育对于伴生体的形成至关重要,但很难在胚胎中直接研究.
- 以前从多能干细胞 (PSC) 生成PGC类细胞 (PGCLC) 的方法依赖于细胞因子尾酒.
- 转录因子SOX17和PRDM1是关键的PGC指标,但它们的平衡表达对生殖细胞命运和内皮分化至关重要.
研究的目的:
- 用SOX17和PRDM1.1的均衡诱导来描述PGCLC分化的详细协议.
- 为研究人类PGC发育及其潜在功能障碍提供一个可访问的实验平台.
- 为了使其他哺乳动物物种的PGCLC诱导.
主要方法:
- 使用了人类多能干细胞 (PSC).
- 诱导PGC的表达以平衡的方式指定SOX17和PRDM1.
- 在不需要细胞因子尾酒的情况下,优化了差异化协议.
主要成果:
- 通过平衡的SOX17和PRDM1诱导,从PSC中成功生成PGCLC.
- 证明这种方法绕过了BMP和其他细胞因子的要求.
- 展示了该协议对其他具有SOX17表达PGC的哺乳动物物种的适用性.
结论:
- SOX17和PRDM1的平衡诱导为PGCLC生成提供了一个强大的方法.
- 该协议促进了胚胎细胞生物学研究及其在生殖技术中的应用.
- 该方法为了解PGC发展和潜在的相关疾病提供了有价值的工具.
相关概念视频
Induced Pluripotent Stem Cells
22.9K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
22.9K
Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Embryonic Stem Cells
3.5K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
3.5K
Chromatin Modification in iPS Cells
1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K


