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perfluorooctanesulfonic 酸 (PFOS) 在正在发育的小鼠甲状腺中破坏了cadherin-16
Nichlas Davidsen1, Louise Ramhøj1, Anne-Sofie Ravn Ballegaard1
1National Food Institute, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.
Current research in toxicology
|February 14, 2024
概括
暴露在 perfluorooctanesulfonic 酸 (PFOS) 中会改变发育大鼠甲状腺的甲状腺基因表达. 这种干扰,特别是在Cdh16表达中,表明对甲状腺发育和激素合成的潜在影响.
科学领域:
- 内分泌学 在内分泌学.
- 发展生物学 发展生物学
- 毒理学 毒理学 毒理学
背景情况:
- perfluorooctanesulfonic 酸 (PFOS) 是一种持久的环境污染物.
- 孕期暴露于PFOS可能会破坏甲状腺激素 (TH) 稳态,并影响发育.
- 以前的研究表明,PFOS可能会影响甲状腺发育.
研究的目的:
- 为了研究PFOS对老鼠甲状腺发育的直接影响.
- 检查PFOS暴露后与甲状腺发育和功能相关的基因表达的变化.
主要方法:
- 利用老鼠甲状腺的活体培养系统.
- 暴露于不同度的PFOS (1微米和10微米) 的甲状腺组织.
- 使用RT-qPCR和RNAscope分析了关键的甲状腺相关基因 (例如Fox1,Pax8,Cdh16,Tpo,LRp2) 的基因表达.
主要成果:
- 暴露于PFOS并没有显著改变甲状腺毛囊结构或大小.
- 观察到Foxe1,Tpo和Lrp2的升调.
- 关键转录因子Pax8及其向基因Cdh16的显著下调.
- 在转录和蛋白质水平上,Cdh16的受损,不整的表达模式,与对照组中均的表达形成鲜明对比.
结论:
- 暴露于PFOS会影响甲状腺基因表达,特别是降低Pax8的调节和改变Cdh16表达模式.
- 干扰Cdh16表达表明对卵泡生长和完整性的潜在影响.
- 这些分子变化可能导致在暴露于PFOS的个体中观察到的甲状腺激素合成受损.
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