有效的自然等离子体转化Vibrio natriegens使零资本分子生物学成为可能
David A Specht1, Timothy J Sheppard1, Finn Kennedy1
1Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA.
PNAS nexus
|February 14, 2024
概括
自然有能力的Vibrio natriegens细胞可以在一个最小的介质中创建,转换和恢复. 这种高效,低成本的方法可以为合成生物学应用提供快速,可扩展的基因工程.
科学领域:
- 微生物学 微生物学
- 合成生物学 合成生物学
- 遗传学 是一个遗传学.
背景情况:
- * *Vibrio natriegens*是一种快速生长的细菌,具有自然能力的能力.
- *自然转化允许细胞在生理条件下吸收外部DNA.
- *现有的转换方法往往需要复杂的程序和多个介质的变化.
研究的目的:
- * 开发一种简化和高效的自然转化协议,用于*自然菌*.
- * 创建一个单介质系统,用于细胞能力,转化和恢复.
- * 建立V. natriegens作为合成生物学成本效益和可扩展的底盘.
主要方法:
- * 设计了一个*V. natriegens*菌株,使用了*Vibrio cholerae*能力调节器*tfoX*.
- * 开发了一种使用乙酸作为能源的最小能力介质 (MCM).
- * 对等离子体进行了转换,线性DNA重组,并在同一介质中进行了共同转换.
主要成果:
- * 已经证明了对等离子体转换,线性DNA重组和共转换的自然能力.
- *通过室温或基于化器的协议实现了高的转换效率 (10^4到10^6 cfu/μg).
- *转化细胞在冷和解后仍然具有能力,并在8小时内产生殖民地.
结论:
- * 开发的协议提供了一种简单,廉价和可扩展的方法,用于自然转化V. natriegens*.
- * 具有自然能力的 *V. natriegens* 可以被用作合成生物学的高效底盘,可能取代 *Escherichia coli*.
- *单介质,室温过程是自动定向进化和大规模应用的理想选择.
相关概念视频
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01:33Bacterial Transformation
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
01:26Transformation
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