NF-κB p65和TCF-4的相互作用与巨细胞的LPS刺激的IL-6分泌有关

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Biochemistry and biophysics reports

|February 14, 2024

概括

这项研究揭示了人类巨细胞中NF-κB和Wnt/β-catenin通路之间的积极交叉,这对于调节炎症期间的互白素-6 (IL-6) 生产至关重要.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

像IL-6这样的促炎细胞因子是宿主防御和炎症的关键.
NF-κB和Wnt/β-catenin通路参与IL-6的产生,细胞类型之间的相关性各不相同.
在巨细胞中,这些通路之间的交叉连接仍然不太清楚.

研究的目的:

研究人类巨细胞中NF-κB和Wnt/β-catenin通路之间的相互作用.
阐明这种交叉在介质素-6 (IL-6) 生产中的作用.

主要方法:

人类巨细胞用脂聚糖 (LPS) 刺激.
使用了特定途径抑制剂 (NF-κB的LY294002,Wnt/β-catenin的PNU74654).
测量了IL-6的分泌水平.
分析了NF-κB p65和TCF-4的核转位和蛋白质相互作用.

主要成果:

在人类巨细胞中观察到NF-κB和Wnt/β-catenin通路之间的 IL-6 生产的正交叉调节.
抑制任何一种途径显著降低了IL-6分泌,即使在LPS刺激下.
LPS刺激诱导NF-κB p65核迁移和与TCF-4相互作用.

结论:

NF-κB和Wnt/β-catenin通路在人类巨细胞中表现出积极的交叉,显著影响IL-6的产生.
这种相互作用通过路径特定的抑制剂得到了功能验证.
NF-κB p65与TCF-4的相互作用是炎症反应期间这种交叉交流的关键事件.

关键词:

炎症性细胞因子脂多多糖类的糖化物巨细胞是一个巨细胞.通过NF-κB信号通路传递信号.在Wnt/β-catenin信号通路中.

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