相关实验视频

Updated: Jul 3, 2025

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

一个通用平台,通过单循环来准MHC-II抗原

Haotian Du¹, Jingjia Liu², Kevin M Jude³

¹Department of Chemistry, Stanford University, Stanford, CA, USA.

bioRxiv : the preprint server for biology

|February 14, 2024

概括

一个新的蛋白质平台,TRACeR-II,能够快速开发特定的MHC-II结合剂以向抗原. 这种多功能系统克服了开发高亲和度,抗原特异性MHC-II向系统的挑战.

科学领域:

免疫学免疫学免疫学
蛋白质工程是指蛋白质工程.
计算生物学计算生物学

背景情况:

主体组织相容性复合物II类 (MHC-II) 分子对于CD4+T细胞和抗原呈现细胞 (APC) 通信至关重要.
由于MHC-II的结构特征,开发具有高亲和度和抗原特异性的向系统具有挑战性.

研究的目的:

引入一个新的蛋白质平台,TRACeR-II,用于快速开发特定的MHC-II结合剂.
为了证明TRACeR-II在抗原识别和结合方面的多功能性和有效性.

主要方法:

开发TRACeR-II蛋白质平台,采用小型螺旋捆架和单环抗原识别机制.
在多个MHC-II等位基因中,TRACeR-II结合剂的快速演变.
计算式蛋白质设计用于针对SARS-CoV-2的特定结合序列的应用.

主要成果:

快速发展的TRACeR-II结合剂用于特异性的MHC-II识别.
该平台在不同的MHC-II等位基因中展示了多功能性.
计算设计成功生成了SARS-CoV-2的特定结合剂,即使具有未知的复杂结构.

结论:

TRACeR-II提供了一种简单而直接的方法来克服MHC向挑战,绕过传统的CDR循环选择.
这一平台对免疫反应调节和多样化的超神应用具有前景.

更多相关视频

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

相关概念视频

Antigen Processing Pathways

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...