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全球释放的翻译性压制横跨Plasmodium的宿主向载体传播事件

Kelly T Rios^1,2, James P McGee^1,2, Aswathy Sebastian³

¹Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802.

bioRxiv : the preprint server for biology

|February 14, 2024

概括

疟疾寄生虫使用蛋白质复合体 (DOZI/CITH/ALBA) 控制基因表达,以确保成功传播. 这项研究揭示了这种复杂的结构是如何形成的.

科学领域:

分子寄生虫学分子寄生虫学
翻译控制翻译控制
菌生物学的生物学

背景情况:

疟疾寄生虫需要精确的基因调节才能在宿主之间传播.
雌性性质细胞储存被抑制的mRNA用于胚胎发育.
对于翻译压制来说,DOZI/CITH/ALBA复合体至关重要.

研究的目的:

调查疟疾寄生虫传播期间mRNA转化抑制的全球释放.
描述DOZI/CITH/ALBA综合体的空间和组成动态.
阐明控制寄生虫传播的分子机制.

主要方法:

对比的转录组学和定量DIA-MS的Plasmodium yoelii的蛋白组学.
基于TurboID的近距离蛋白质组学来研究蛋白质复杂相互作用.
超结构膨胀显微镜和结构化照明显微镜用于空间分析.
蛋白组学用于识别蛋白质修饰.

主要成果:

几乎有200个副本被释放用于翻译后受精,其中一些被压抑.
DOZI/CITH/ALBA复合体的蛋白质在压缩释放后在囊细胞中解离.
DOZI相互作用将从NOT1-G切换到NOT1,可能触发mRNA释放.
关键的翻译控制蛋白在胚胎中被修改.

结论:

在疟疾寄生虫传播期间释放全球转化抑制的详细模型.
确定了控制寄生虫发育的关键分子事件和蛋白质动态.
提供了对疟疾寄生虫宿主向载体传播的基本机制的见解.

关键词:

美国防空局蛋白质组学疟疾传播疟疾传播接近性蛋白质组学接近性蛋白质组学在RNA-seqqq.翻译的压制翻译的压制

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