scCensus:目标外的scRNA-seq读数揭示了有意义的生物学
Dongze He1,2, Stephen M Mount3, Rob Patro1,4
1Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
bioRxiv : the preprint server for biology
|February 14, 2024
概括
单细胞RNA测序 (scRNA-seq) 中的非目标读取包含有价值的生物信息. 分析这些读数可以改善基因检测,揭示染色质结构,并增强细胞相似性识别.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 单细胞RNA测序 (scRNA-seq) 对于理解细胞异质性至关重要.
- 很大一部分scRNA-seq读数可能来自"目标之外"的来源,而不仅仅是多基化RNA的3'末端.
结论:
- 目标之外的scRNA-seq读取对转录活动的低估信息.
- 这些发现鼓励开发新的算法和方法,利用非目标读数进行更准确的单细胞分析.
更多相关视频
06:24Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
Published on: March 12, 2021
3.6K
07:35Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
Published on: December 1, 2023
672
相关概念视频
RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
