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通过微藻发酵开发和大规模生产人乳脂肪模拟物
Xiaoying Zhou1, Xinhua Zhao1, Leon Parker1
1Checkerspot, Alameda, CA, United States.
Frontiers in nutrition
|February 14, 2024
概括
这项研究开发了一种新的发酵方法,使用微藻生产母乳三糖醇 (TAG). 这个过程成功地创造了OPO TAG,这对婴儿营养至关重要,证明了工业应用的可扩展性.
科学领域:
- 生物技术是生物技术.
- 营养科学 营养科学
- 微生物学 微生物学
背景情况:
- 母乳含有复杂的三糖醇 (TAG),很难用常见的成分复制.
- 重建特定的TAG结构对于婴儿营养配方至关重要.
研究的目的:
- 开发一种创新的发酵技术,生产必要的人母乳TAG.
- 为了克服复制母乳脂肪成分用于婴儿配方奶粉的挑战.
主要方法:
- 使用微藻油平台,对脂肪酸和TAG成分进行两步修改过程.
- 采用经典菌株改进来增加棕酸 (16:0) 并表达了lysophosphatidic acid acyltransferase (LPAAT) 基因用于sn-2棕酸合并.
- 将发酵量扩大到50L,然后进行石油提取,提炼和使用液态染色体质谱学分析.
主要成果:
- 通过菌株改进,将总16:0从25%提高到30%.
- 在LPAAT表达后,获得了sn-2棕酸的十倍增加,达到LPAAT表达后的总棕酸的73%.
- 证实了主要的TAG物种为1,3-dioleoyl-2-palmitoyl-glycerol (OPO),并在5天内证明了可扩展的产量>150g/L.
结论:
- 成功证明了生产具有特定 OPO 结构的母乳 TAG 的可行性.
- 开发的发酵过程是可扩展的,适合整合到婴儿营养配方中.
- 这项创新解决了婴儿营养的重大障碍,提供了必要的乳脂的来源.
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