在哈萨克斯坦马的卵巢颗粒细胞的差异年龄相关的转录组分析
Wanlu Ren1, Jianwen Wang1,2, Yaqi Zeng1
1College of Animal Science, Xinjiang Agricultural University, Urumqi, China.
Frontiers in endocrinology
|February 14, 2024
概括
老龄化哈萨克马的生殖能力下降与卵巢颗粒细胞的分子变化有关. 本研究确定了参与毛囊发育和衰老的关键调节网络和途径.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
背景情况:
- 哈萨克马因早期成熟和季节性而闻名.
- 在这种品种中,生殖能力在14岁后显著下降.
研究的目的:
- 研究哈萨克马的生殖衰老背后的分子机制.
- 在不同年龄组的卵巢粒状细胞中识别不同表达的基因和调节网络.
主要方法:
- 来自不同年龄 (1,2,7和>15岁) 的哈萨克马的卵巢颗粒细胞的整体转录组测序.
- 生物信息分析包括差异表达分析,基因本体学 (GO) 丰富,基因和基因组的京都百科全书 (KEGG) 途径分析和蛋白质-蛋白质相互作用 (PPI) 网络构建.
- 建立一个长非编码RNA (lncRNA) -微RNA (miRNA) -信使RNA (mRNA) 的竞争性内源RNA (ceRNA) 网络.
主要成果:
- 随着年龄的增长,在颗粒细胞中观察到mRNA,lncRNA,miRNA和circRNA表达特征的显著变化.
- 发现的关键途径包括MAPK,Hippo,Wnt,信号,阿尔多合成,细胞衰老和NF-kappa B信号.
- 建立了一个涉及lncRNA-miRNA-mRNA的差异性调节网络,这对细胞增殖,分化和细胞亡至关重要.
结论:
- 这项研究阐明了哈萨克马的卵泡发育和生殖衰老的分子机制.
- 鉴定到的调节网络为了解马的卵巢发育和衰老提供了理论基础.
- 这些发现突显了马匹生殖过程中特定信号通路的重要性.
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