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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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相关实验视频

Updated: Jul 3, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

放大传感能力:通过多变量分析结合等离子体共振和弗雷内尔反射.

Jaione Etxebarria-Elezgarai¹, Luca Bergamini^2,3, Eneko Lopez^1,4

¹CIC nanoGUNE BRTA, Tolosa Hiribidea 76, San Sebastian, 20018, Spain.

|February 14, 2024

概括

这项研究通过使用金纳米光盘和机器学习来提高生物传感性能. 这种创新方法显著提高了分析灵敏度和传感器分辨率,以改善检测.

关键词:

弗雷斯内尔的反射是新鲜的克雷奇曼配置的配置.局部化的表面等离子体多变量分析多变量分析.部分最小正方形.等离子体纳米结构的结构.等离子体表面格子共振.

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Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

相关实验视频

Last Updated: Jul 3, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

科学领域:

纳米技术纳米技术
生物感应是一种生物感应.
光学是什么?光学是什么?光学是什么?

背景情况:

多变量分析通过提取关键信息和减轻噪音来改善生物传感.
等离子传感器为检测介电变化提供高灵敏度.

研究的目的:

为了提高生物传感性能,使用金纳米光盘作为Kretschmann设置中的元表面.
利用机器学习和多变量分析来提高分析灵敏度和分辨率.

主要方法:

使用金纳米光盘作为一个Kretschmann配置中的 metasurface.
采用机器学习来分析反射率曲线中的特征.
整合了超表面与微流体室,以结合弗雷内尔反射.

主要成果:

工程超表面产生了超出经典克雷茨曼共振的新型等离子体特征.
集成装置提供了来自弗雷内尔反射的额外光谱特征.
多变量分析使分析灵敏度提高了200%以上,传感器分辨率提高了20%,预测误差减少了40%.

结论:

塑元表面和弗雷内尔反射的组合显著提高了生物感知能力.
这种方法提供了可用于通常可用的设置的增强检测性能.