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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

使用磁性激活的连续屈曲微流体芯片动态选择高亲和度的阿普坦体.

Ke-Zhu Yang¹, Meng Wang¹, Ming-Yue Gao¹

¹College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China. zlzhang@whu.edu.cn.

Chemical communications (Cambridge, England)

|February 14, 2024

概括

一个新的磁激活连续偏移 (MACD) 芯片使得高效的aptamer选择. 这种动态选择方法迅速丰富了高亲和度的体,正如Candida albicans的一个特定体的分离所证明的那样.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
分析化学分析化学

背景情况:

在分子诊断和治疗方面,aptamer的发现至关重要.
传统的aptamer选择方法可能耗时且效率低下.
开发更快,更有效的aptamer选择技术至关重要.

研究的目的:

设计和验证磁激活连续偏移 (MACD) 芯片,用于加速的阿帕特默发现.
为了证明动态选择在丰富高亲和度体中的有效性.
为了获得对Candida albicans的高亲和度和高特异性的吸收体.

主要方法:

设计了一个MACD芯片,以促进连续流动动态选择.
使用MACD芯片在六轮中对Candida albicans进行了选择.
通过调整目标度来调整选择严格度.
使用解离常数 (Kd) 量化了阿普塔默的结合亲和力.

主要成果:

在连续流系统中,MACD芯片使连续绑定和分离成为可能.
动态选择有效地解离了低亲和度序列,并丰富了高亲和度的体.
成功分离出一种C.al3胺体,它对Candida albicans具有很高的亲和力和特异性.

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选择的阿胺体表现出 7.9 nM 的解离常数 (Kd).

结论:

使用MACD芯片进行动态选择是快速发现aptamer的高效策略.
开发的MACD芯片技术可以加速识别高亲和度的体.
孤立的C.al3胺体对向Candida albicans的应用有希望.