相关实验视频
Updated: Jul 3, 2025

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
Published on: May 17, 2019
由血液传播的病原体引发的系统性炎症反应综合征诱导长时间的树突细胞和免疫抑制
Mitra Ashayeripanah1, Javier Vega-Ramos2, Daniel Fernandez-Ruiz3
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC 3000, Australia.
系统性炎症反应综合征 (SIRS) 通过使常规树突细胞 (cDCs) 而导致免疫抑制. 这种会影响抗原呈现和免疫力数周,但可以通过向治疗来克服.
科学领域:
- 免疫学 免疫学 免疫学
- 病原体诱导的免疫反应
- 细胞免疫学 细胞免疫学
背景情况:
- 血液传播的病原体可以触发全身炎症反应综合征 (SIRS),导致严重的,可能致命的免疫抑制.
- 导致SIRS后免疫损伤的潜在机制尚未完全理解.
研究的目的:
- 为了阐明导致SIRS后免疫功能障碍的机制.
- 确定细胞点和策略,在SIRS后恢复免疫能力.
主要方法:
- 在实验模型中使用病原体模仿或疟疾感染来诱导SIRS.
- 分析传统树突细胞 (cDC) 功能,表型和转录组学.
- 通过cDCs进行抗原捕获和呈现的体内评估.
- 评估治疗干预措施,包括单克隆抗体和TGF-β阻断.
主要成果:
- 在常规树突细胞 (cDCs) 中,SIRS诱导功能性,影响多个细胞代,持续3-4周.
- 的cDC表现出明显的分子特征,抗原呈现能力受损,细胞因子形状改变.
- cDC麻起源于外围组织的末端分化过程中,受到SIRS解析后持续信号的影响.
- 准cDC受体或阻断TGF-β可以部分恢复免疫功能并减少免疫抑制.
结论:
- 对cDCs的功能性是SIRS后免疫抑制的关键机制.
- 在分化过程中,cDC是外部诱导的,并在SIRS解决后持续存在.
- 针对cDC或相关信号通路的治疗策略显示出恢复免疫能力的希望.
更多相关视频
09:04Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
11:48Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Published on: May 31, 2018
相关概念视频
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Cell-mediated Immune Responses
Humoral Immune Responses
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...