综合转录基因和蛋白质基因分析确定了与葡萄中植物生长调节剂诱导的度相关的新型调节基因
Jiao Feng1, Wen Zhang2, Wu Wang3
1College of Horticulture, Sanya Institute of Nanjing Agricultural University (NJAU), Nanjing 210095, China.
Journal of agricultural and food chemistry
|February 14, 2024
概括
植物生长调节剂,如CPPU和GA3,通过增加proanthocyanidin水平来增强葡萄的性. 基因表达分析确定了关键调节者,如VvWRKY71,参与了这个过程.
科学领域:
- 植物科学 植物科学
- 食品科学 食品科学 食品科学
- 遗传学 遗传学 是一个
背景情况:
- 收缩性是食用葡萄的关键感官属性,显著影响味道和质量.
- 了解的分子基础对于葡萄品种和种植至关重要.
研究的目的:
- 调查植物生长调节剂 (CPPU和GA3) 对"夏季黑"葡萄中的感官收缩性和益西安丁 (PA) 度的影响.
- 为了确定分子机制和调节基因参与植物生长调节器诱导的性.
主要方法:
- 葡萄的感官评估. 葡萄的感官评估.
- 在葡萄皮中的flavan-3-ols/proanthocyanidins (PAs) 的量化.
- 综合转录和蛋白质组分析.
- 在葡萄叶和 *Nicotiana benthamiana* 中进行过度过度表达和促进子测试.
主要成果:
- CPPU和GA3的应用显著增加了葡萄中的感觉度和PA度.
- 转录基因和蛋白质基因分析揭示了与黄类生物合成相关的不同表达的基因和蛋白质.
- 特定的基因,包括VvWRKY71,VvMYBPA1,VvMYC1和VvNAC83,被确定为PA生物合成的调节者,VvWRKY71和VvNAC83显示复杂的调节作用.
结论:
- 植物生长调节剂CPPU和GA3可以通过影响PA积累来调节葡萄中的感觉度.
- 关键的调节基因,特别是VvWRKY71和VvNAC83,在控制PA生物合成,从而控制度方面发挥着重要作用.
- 这项研究为葡萄中植物生长调节器介导的缩作用背后的分子通路提供了新的见解.
关键词:
在CPPU中使用.GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3GA3黄-3-ol的使用方法氨基氨基的含量是多少感官分析是一种感官分析.更多相关视频
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