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光探针的设计和应用,以检测细胞物理微环境

Junbao Ma^1,2,3, Rui Sun^4,5, Kaifu Xia^1,2,3

¹Department of Chemistry and Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou 310030, Zhejiang Province, China.

Chemical reviews

|February 14, 2024

概括

小分子光探测器可以可视化细胞微环境. 这些探头测量pH和粘度等物理参数,帮助诊断疾病和理解生物过程.

科学领域:

生物化学和生物物理学
化学生物学化学生物学
细胞生物学细胞生物学

背景情况:

细胞微环境的物理特性显著影响细胞生理学和病理学.
精确测量微环境波动需要时空分辨率.
光探头为生物应用提供灵敏,动态的检测.

研究的目的:

审查用于测量细胞微环境物理参数的小分子光探针.
为了将化学机制与各种参数的光信号变化相关联.
为开发先进的光探测器提供视角,用于生物洞察和诊断.

主要方法:

专注于用于检测微环境参数的小分子光探针.
描述化学机制,将物理性质与光信号联系起来.
审查从体外样本到活体动物模型的申请.

主要成果:

小分子光探头可以测量pH值,极性,粘度,机械力,温度和电子电位.
光信号与特定的物理性质变化相关.
探测器有助于在各种生物环境中进行动态检测.

结论:

小分子光探针是可视化动态细胞微环境的宝贵工具.
这些探测器提高了对生物过程的理解.
这些探针的开发可以丰富人类疾病的诊断工具.

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