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相关概念视频

Non-Canonical Wnt Signaling Pathways01:41

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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相关实验视频

Updated: Jul 3, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
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玉米活性脱聚合因子1 (ZmADF1) 负面调节花粉发育.

Guihua Lv1, Yunfeng Li2, Zhengxin Wu1

  • 1Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310004, China.

Biochemical and biophysical research communications
|February 14, 2024
PubMed
概括
此摘要是机器生成的。

ZmADF1基因负面调节玉米花粉的数量和活力. 淘汰ZmADF1提高了花粉的生存能力,为提高玉米育种和谷物生产提供了途径.

关键词:
乙烯酸脱聚合因子1的作用因子1基因编辑 基因编辑玉米 玉米 玉米 是 一种过度表达是一种过度表达.关于花粉的发展

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Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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科学领域:

  • 植物生物学 植物生物学
  • 分子遗传学 分子遗传学
  • 农业科学 农业科学

背景情况:

  • 花粉的发育和受精对于血管精子的繁殖和作物产量至关重要.
  • 动氨酸脱聚合因子 (ADF) 蛋白通过与动氨酸相互作用来调节重要的植物过程.
  • 了解ADF基因功能对于改善作物育种策略至关重要.

研究的目的:

  • 阐明ZmADF1基因在玉米花粉发育和繁殖中的功能.
  • 研究ZmADF1在调节花粉数量,活力和发芽中的作用.
  • 探索ZmADF1作为改善玉米育种的目标的潜力.

主要方法:

  • 生物信息分析和ZmADF1.1.的亚细胞定位.
  • 在阿拉比多普西斯和玉米中基因过度表达.
  • 在玉米中进行CRISPR/Cas9介导基因淘汰.
  • 评估转基因和淘汰线的花粉特征和种子设置率.

主要成果:

  • ZmADF1显示出高序列保存,并且局部存在于核和细胞质中.
  • ZmADF1 特别表达在玉米花粉中.
  • 过度表达ZmADF1减少了Arabidopsis中的花粉数量,并对花粉发芽和种子集产生了负面影响.
  • 在玉米中,ZmADF1淘汰线表现出显著增强的花粉活力.

结论:

  • ZmADF1负面调节花粉量,活力,发芽和种子设置速度.
  • 准ZmADF1为改善玉米繁殖和产量提供了一个潜在的战略.
  • 这项研究为植物繁殖中的ADF基因功能提供了宝贵的见解.