易于制备嵌入的含有极群的五甲静止相,用于高度选择性地分离各种分析物
Abul K Mallik1, Lidia Montero2, Sven W Meckelmann3
1Applied Analytical Chemistry, University of Duisburg-Essen, Universitaetsstr. 5, 45141 Essen, Germany; Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka 1000, Bangladesh.
合成了三种新的五二 (PFP) 静止相,嵌入了极性群. 这些新的PFP相在逆相 (RPLC) 和水友相互作用 (HILIC) 液态染色学中提供了增强的选择性.
科学领域:
- 分析化学 分析化学
- 染色学科学 染色学科学
背景情况:
- 五甲 (PFP) 静止相在染色学中至关重要,仅次于C18相.
- 嵌入式极点组 (EPG) 可以在静止相中引入额外的相互作用点,从而潜在地提高色谱性能.
研究的目的:
- 综合和描述包含EPG的新型PFP静止阶段.
- 评估这些新相在逆相液态染色学 (RPLC) 和水友相互作用染色学 (HILIC) 两种新相的性能.
主要方法:
- 用 (3-aminopropyl) trimethoxysilane (APS) 修改,然后与不同的五二衍生物合.
- 使用元素分析,ATR-FTIR,SEM和TGA进行表征.
- 使用RPLC和HILIC模式的Tanaka和Neue测试对色谱性能进行评估.
主要成果:
- 三个新的阶段 (Sil-PFP-BA,Sil-PFP-AA,Sil-PFP-AN) 已成功合成和表征.
- 在RPLC中,Sil-PFP-AA表现出卓越的分子形状选择性.
- 与商业PFP列相比,Sil-PFP-AN在HILIC中表现出最高的分离能力.
- Sil-PFP-AA被应用于 (RPLC) 中的类类分析.
- Sil-PFP-AN被应用于维生素C分析 (HILIC).
结论:
- 新合成的含EPG的PFP静态相对于特定的染色学应用具有明显的优势.
- Sil-PFP-AA在RPLC中的形状选择性分离中是有效的,适合分析素.
- 西尔-PFP-AN对HILIC分离有希望,由维生素C分析证明.
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