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乳酸盐通过调解ROS/p38 MAPK信号通路来诱导C2C12神经细胞的分化

Chunfang Cheng¹, Wenxi Li², Yuanqian Ye²

¹School of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China.

|February 14, 2024

概括

乳酸盐通过活性氧物种 (ROS) 和p38 MAPK通路促进骨肌细胞的分化. 这项研究揭示了乳酸盐.

科学领域:

肌肉生物学肌肉生物学
细胞信号传递细胞信号传递
生物化学生物化学

背景情况:

乳酸盐被认为是骨肌肉的能量基质.
新出现的证据表明乳酸在肌体分化中的调节作用.
乳酸盐在肌肉细胞过程中影响反应性氧物种 (ROS) 水平.

研究的目的:

为了研究乳酸盐对C2C12核细胞分化的影响.
阐明ROS和p38 MAPK信号传导在乳酸介导肌体发生中的作用.
了解乳酸,ROS和p38 MAPK在骨肌肉分化中的相互作用.

主要方法:

在体外分化研究中利用C2C12核细胞.
在生理度下服用乳酸,以评估其影响.
作为ROS清除剂和SB203580作为p38 MAPK抑制剂使用的n-乙半氨酸 (NAC).
监测的肌原分化标志物 (Myf5,MyoD,MyoG) 和p38 MAPK酸化.

主要成果:

乳酸盐显著增强了C2C12神经细胞分化,以剂量依赖的方式.
乳酸诱导的分化伴随着ROS产量的增加.
NAC预处理减弱了乳酸对分化和p38 MAPK酸化的影响.
SB203580预处理抑制了乳酸诱导的C2C12核细胞分化.
发现乳酸诱导的ROS生成调解了p38 MAPK激活.

结论:

乳酸盐通过一种依赖ROS的机制促进C2C12髓细胞分化.
这种p38 MAPK信号通路对于乳酸介导肌体生成至关重要.
乳酸在肌肉发育中的有益作用涉及ROS敏感通道.
研究结果表明,ROS通过乳酸信号传递对骨肌肉功能产生积极影响.

关键词:

在C2C12神经母细胞.乳酸盐是一种乳酸盐.肌源性差异化的分化在P38 MAPKK中使用.这就是ROSOS ROS.

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