减少了人类鼻粘膜表皮缺乏ZNF36565的黄金葡萄球菌的增殖能力和防御能力
Xiaoyun Du1, Longgang Yu2, Lin Wang2
1Department of Otolaryngology, Head and Neck Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China, dxyun030509@163.com.
International archives of allergy and immunology
|February 14, 2024
概括
指蛋白ZNF365对于鼻上皮细胞的健康和对黄金葡萄球菌的防御至关重要. 减少ZNF365与长期鼻炎与鼻息肉 (CRSwNP) 的发展有关.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
背景情况:
- 带鼻息肉的慢性鼻炎 (CRSwNP) 涉及鼻障碍功能障碍.
- 指蛋白365 (ZNF365) 在CRSwNP病变发生中的作用尚未被探索.
- 研究ZNF365对鼻上皮细胞增殖和对黄金葡萄球菌 (Staphylococcus aureus) 的防御的影响至关重要.
研究的目的:
- 研究ZNF365在鼻膜上皮细胞增殖中的作用.
- 为了确定ZNF365对上皮细胞防御对金黄色葡萄球菌 (S. aureus) 感染的作用.
- 探索ZNF365在CRSwNP的发展中的潜在参与.
主要方法:
- 经验证的ZNF365表达在鼻息肉组织和初级上皮细胞的变化,使用免疫组织化学和西欧涂抹.
- 在人类鼻上皮细胞系 (HNEpc) 中利用ZNF365敲击来评估通过免疫光学,QPCR,CCK8和细胞测试的增殖,迁移和分化.
- 在S. aureus刺激后分析了介质细胞标记物和TLR4-MAPK-NF-κB通路的变化.
主要成果:
- 与对照组相比,ZNF365的表达在鼻息肉组织和原发性上皮细胞中显著降低.
- ZNF365 knockdown 损害了上皮细胞的增殖,迁移和分化,由减少的紧结蛋白表达体现出来.
- 黄金菌感染加剧了这些影响,抑制了增殖和迁移,同时增加了上皮细胞-介质细胞过渡和炎症标志物.
结论:
- ZNF365在维持鼻膜上皮细胞的增殖和防御S. aureus的过程中发挥着至关重要的作用.
- 减少ZNF365表达与表皮屏障功能受损和对S. aureus的敏感性增加有关.
- 这些发现表明,ZNF365可能是CRSwNP.病变发生的关键因素.
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