在生物流体中通过基于电化学体的生物传感器快速纳米分子检测Δ9-四大麻
Yu Xie1, Jin-Ping She2, Jia-Xing Zheng2
1Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China; College of Chemistry and Chemical Engineering, Jinggangshan University, Ji'an 343009, China.
Analytica chimica acta
|February 14, 2024
概括
这项研究介绍了一种新的传感器,用于快速,灵敏地检测生物流体中的delta-9-tetrahydrocannabinol (THC). 开发的基于电化学体 (EAB) 的电化学传感器为吸毒驾驶的查提供了准确的结果.
科学领域:
- 电化学 电化学 电化学
- 生物传感器是一种生物传感器.
- 分析化学 分析化学
背景情况:
- 开发用于复杂生物流体的快速,灵敏和选择性传感器对于现实世界的应用至关重要.
- 吸毒驾驶路边查需要快速检测毒品的方法,最小的矩阵干扰.
- 在电极接口上最小化生物流体矩阵干扰是提高传感器灵敏度的关键.
研究的目的:
- 开发一种简单,绿色的方法来制备多孔粗电极.
- 为了构建一个基于电化学的阿普他默 (EAB) 传感器来检测三角-9-四大麻素 (THC).
- 在各种生物流体中实现快速,灵敏和准确的THC检测.
主要方法:
- 制造有多孔的粗电极,具有增加的电活性表面积.
- 使用准备好的电极,构建基于电化学体 (EAB) 的电化学传感器.
- 在未稀释的血清,尿液和唾液样本中测试传感器的性能.
主要成果:
- 与光滑电极相比,粗的电极具有21倍大的电活性面积.
- 粗电极显示出显著改善的防性能.
- 在60秒内,EAB传感器在未稀释血清 (5.0nM),尿液 (10nM) 和唾液 (10nM) 中达到THC的纳米分子检测极限.
- 传感器显示出良好的可重现性,准确性和特异性.
结论:
- 孔隙结构减少非特异性吸附并增强信号稳定性.
- 较大的活性电极区域允许更多的aptamer固定,提高灵敏度.
- 开发的EAB传感器由于其低检测极限和快速测量时间,符合吸毒驾驶查的要求.
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