在长非编码RNA中,Z-RNA的优先形成超过了间隔动图
Uditi Bhatt1, Anne Cucchiarini2, Yu Luo2
1School of Molecular Sciences, The University of Western Australia, Crawley, Western Australia 6009, Australia.
Genome research
|February 14, 2024
概括
长非编码RNAs (lncRNAs) 可以形成非正规结构,如G-四复合体 (G4s) 和Z-RNAs,影响它们的功能. 这项研究揭示了许多lncRNA中的G4和Z-RNA结构,进步了对lncRNA结构-功能关系的理解.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 二级结构对于长非编码RNA (lncRNA) 功能至关重要,作为支架和调解相互作用.
- 非正规的RNA结构,包括G-四重复合体 (G4s),间隔动机 (iMs) 和R-循环 (RLs),在基因调节中起着重要的作用.
研究的目的:
- 通过计算预测和实验验证 lncRNA转录组中的非正规的二次结构.
- 为了比较这些结构在lncRNAs与蛋白质编码转录的流行程度.
- 通过表达和途径分析,评估预测的lncRNA结构的生物相关性.
主要方法:
- 利用计算工具G4-iM Grinder和QmRLFS-finder在lncRNA转录组中进行结构预测.
- 整合了预测结构与lncRNA组织表达数据,并进行了途径分析.
- 采用体外生物物理实验来确认在精选的lncRNA中预测的G4 (pG4) 和iM (piM) 结构的形成.
主要成果:
- 在生物学意义上的lncRNA中确定了许多以前未报告的,高度稳定的G4结构.
- 发现预测的iM (piM) 序列并没有形成iMs,而是意外地形成了Z-RNA结构.
- 首次证明了lncRNA中G4和Z-RNA结构的形成.
结论:
- 非正规的二次结构,特别是G4s和Z-RNAs,在lncRNA中很普遍,可能有助于它们的多样化功能.
- 在C丰富的lncRNA区域中发现Z-RNA结构为研究蛋白质-RNA相互作用开辟了新的途径.
- 这项工作显著增强了对lncRNAs结构-功能范式的理解.
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