相关实验视频

Updated: Jul 3, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

长非编码RNA H19:调节癌症细胞死亡的功能和机制

Yuyang Xia¹, Tianjiao Pei^2,3, Junjie Zhao¹

¹Department of Urology, Institute of Urology, West China Hospital, West China School of Medicine, Sichuan University, 610041, Chengdu, China.

Cell death discovery

|February 14, 2024

概括

长非编码RNA H19 (H19) 抑制癌细胞死亡,促进瘤生长和化学抵抗. 了解 H19 的理解

科学领域:

分子生物学分子生物学
癌症研究癌症研究
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

长非编码RNAs (lncRNAs) 越来越多地与癌症的发展有关.
LncRNA H19 (H19) 是一种已知参与瘤进展的瘤基因.
新出现的证据强调了H19在抑制癌细胞编程细胞死亡 (PCD) 中的作用.

研究的目的:

审查H19调节癌症编程细胞死亡 (PCD) 的机制.
探索H19参与各种信号通路,分子机制和与PCD相关的表观遗传修饰.
突出H19作为癌症治疗中的潜在治疗点的重要性.

主要方法:

文献综述总结了有关H19和PCD癌症的现有研究.
通过信号通路 (例如,Wnt/β-catenin,PI3K-Akt-mTOR) 对H19的调节作用的分析.
检查H19作为竞争性内源RNA (ceRNA) 的功能及其与RNA结合蛋白 (RBPs) 的相互作用.
对影响H19介导PCD的表观遗传修饰 (DNA甲基化,基因组修饰) 的研究.

主要成果:

通过多个分子和信号通路,H19积极抑制癌细胞PCD.
H19作为ceRNA的功能,与RBPs相互作用以调节亡.
表观遗传修饰在H19对PCD的调节中起着至关重要的作用.

结论:

H19是癌症中PCD的关键调节者,影响瘤发育和化学抵抗.
H19代表了癌症治疗的有前途的治疗标.
对H19多方面的作用的进一步研究将促进癌症治疗策略的发展.

更多相关视频

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

The Extrinsic Apoptotic Pathway

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...