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通过MinION纳米孔测序对现场STR类型和人类身份测试的试点验证

Yuan Luo¹, Jiarong Zhang^2,3, Ming Ni⁴

¹Laboratory of Clinical Medicine, Air Force Medical Center, Air Force Medical University, PLA, Beijing, P. R. China.

Electrophoresis

|February 15, 2024

概括

便携式纳米孔测序现在可以在现场识别个体. 这项使用牛津纳米孔技术的技术,使得在事故现场进行法医应用的快速DNA分析成为可能.

科学领域:

法医科学法医科学法医科学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

纳米孔测序为法医应用提供了便携性和实时分析等优势.
目前基于现场的个人识别方法在速度和可访问性方面面临限制.

研究的目的:

为了证明使用纳米孔测序用于现场个体识别的可行性.
评估用于法医DNA分析的便携式纳米孔测序系统的性能.

主要方法:

一个便携式检测盒集成了一个纳米孔MinION MK1B设备用于现场使用.
分析了短串重复 (STR) 位点以进行个体识别.
进行了双盲实验,以验证基因型准确性.

主要成果:

通过分析23个STR位点,在24小时内实现了12个样本的个别识别.
1150种基因型的基因型定型成功率为95.3%,准确率为86.87%.
STR基因造型的准确性与黄金标准方法进行了验证.

结论:

纳米孔测序平台在法医中可用于基于现场的个体识别.
生物信息学和测序技术的进一步进步将提高实时识别能力.
开发的便携式系统显示出在事故现场进行快速DNA分析的前景.

关键词:

在 MiSeq FGx 测序平台上.在MINION纳米孔测序.法医遗传学法医遗传学在现场的识别.短串联重复重复的重复

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