千足动物肠道中的病毒群落:关于多样性的洞察和在调节微生物群中的潜在作用
Julius Eyiuche Nweze1,2, Johannes Sergej Schweichhart1,2, Roey Angel1
1Institute of Soil Biology and Biogeochemistry, Biology Centre CAS, České Budějovice, Czechia.
Environmental microbiology
|February 15, 2024
概括
这项研究揭示了千足动物后中的DNA和RNA病毒,发现了感染大量 prokaryotes 的独特病毒群体. Lysogenic 病毒占主导地位,这表明千足动物微生物组中的 Piggyback-the-Winner 动态.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 生态生态学 生态生态学
背景情况:
- 千足动物是具有复杂肠道微生物组的关键破坏性动物.
- 以前的研究在很大程度上忽视了这些微生物组的病毒成分 (病毒组).
研究的目的:
- 描述两种不同的千足动物物种后肠中的DNA和RNA病毒多样性.
- 为了研究病毒群体和它们的 prokaryotic 主体之间的关系.
主要方法:
- 千足动物后肠内容的元基因组和元转录组测序.
- 病毒基因组的组装和病毒种群的识别.
- 对CRISPR-Cas系统和辅助代谢基因的分析.
主要成果:
- 在热带Epibolus pulchripes和温带Glomeris connexa之间观察到病毒群落的显著差异.
- 病毒优先向每个宿主中最丰富的原生生物种群.
- 常见的DNA病毒包括Caudoviricetes,Cirlivirales和Microviridae;RNA病毒包括Leviviricetes和Potyviridae.
- 在CRISPR-Cas系统和参与基降解和营养生物合成的辅助代谢基因普遍存在.
- 检测到病毒与微生物的比例较低, lysogenic 病毒的流行率较高.
结论:
- 千足病毒组是多样化和宿主特异性的,病毒与丰富的 prokaryotic 成员共同进化.
- 病毒辅助代谢基因可能在宿主代谢中发挥作用,包括基降解.
- lysogeny 的流行支持了"Piggyback-the-Winner" 的生态策略在千足动物肠道病毒中.
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