优化电膜提取中度或低极性的基本物质的通用条件
Chenchen Song1,2, Chen Zhou1,3, Frederik André Hansen1
1Department of Pharmacy, University of Oslo, Oslo, Norway.
Journal of separation science
|February 15, 2024
概括
两种新的通用电膜提取 (EME) 方法,B1和B2,被优化为基本制药化合物. 这些方法为特定极性范围内的分析物提供了高回收率和精度.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
- 生物医学分析 医学分析
背景情况:
- 电膜提取 (EME) 是一种多功能样品制备技术.
- 开发通用的EME方法简化了对各种化合物类别的分析.
- 在等离子体等复杂矩阵中的基本分析物中优化EME对于制药分析至关重要.
研究的目的:
- 开发和优化两个基本分析物的通用电膜提取 (EME) 方法.
- 为每个方法建立不同的极性窗口,以最大限度地恢复分析物.
- 通过在人体血中使用广泛的药物化合物来验证这些方法的性能.
主要方法:
- 两种通用EME方法 (B1和B2) 是使用原型导电瓶EME设备设计的.
- 方法B1使用了2-二乙烯乙烯以太作为分析物的液体膜,Log P为2.06.0.0.
- 方法B2采用2-undecanone作为分析物的液体膜,其日志P为1.04.5.5. 两者都涉及血酸化和提取与特定的潜力和激发.
主要成果:
- 这两种方法都在定义的极性窗口内显示出大多数模型分析物的高回收率 (40%-100%).
- 达到了极好的日内精度,相对标准偏差在2.2%至9.7%之间.
- 在EME系统显示稳定的当前和可接受的矩阵效应值 (90%-109%).
结论:
- 开发的通用EME方法 (B1和B2) 对于从人体血中提取基本药物化合物是有效的.
- 这些方法为制备药品分析样本提供了强大而高效的方法.
- 优化的协议提供了高回收率,良好的精度和稳定性,使它们适合常规分析.
相关概念视频
Extraction: Advanced Methods
447
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
447
Capillary Electrophoresis: Applications
397
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
397
Ion Exchange
592
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
592
Detergent Purification of Membrane Proteins
5.2K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.2K
Optimizing Chromatographic Separations
396
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
396
Osmosis and Osmotic Pressure of Solutions
40.0K
A number of natural and synthetic materials exhibit selective permeation, meaning that only molecules or ions of a certain size, shape, polarity, charge, and so forth, are capable of passing through (permeating) the material. Biological cell membranes provide elegant examples of selective permeation in nature, while dialysis tubing used to remove metabolic wastes from blood is a more simplistic technological example. Regardless of how they may be fabricated, these materials are generally...
40.0K


