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流体调节的脂平衡,涉及脂质体,直到亚微粒大小的组件

Nikita Joseph¹, Marzieh Mirzamani², Tarfah Abudiyah¹

¹Flinders Institute for Nanoscale Science and Technology, College of Science and Engineering, Flinders University Bedford Park SA 5042 Australia colin.raston@flinders.edu.au.

Nanoscale advances

|February 15, 2024

概括

流体装置 (VFD) 能够实现~110nm脂质体的可扩展生产,克服了传统微流体平台的局限性. 这种薄膜装置还可以进行脂质体标签和脂体自组合的现场研究.

科学领域:

生物物理学的生物物理.
材料科学材料科学材料科学
纳米技术纳米技术

背景情况:

传统的微流体平台用于脂质体的形成,但面临着可扩展性的挑战.
脂质体是基于脂的纳米结构,在药物输送和仿生学中具有应用.

研究的目的:

为了研究使用流体装置 (VFD) 进行可扩展的脂质体生产.
在VFD条件下描述脂和脂质体形成的自我组装.
在VFD中探索脂质体行为和在剪切应力下的稳定性.

主要方法:

使用45°倾斜,快速旋转的玻璃管 (VFD) 具有疏水的内部表面,用于连续流处理.
在VFD前脂质体的光剂标记中采用了封闭的操作模式.
在现场进行小角度中子散射 (SANS) 来分析脂组合.

主要成果:

该VFD成功地产生了平均直径约为110纳米的脂质体.
旋转速度的增加导致了~110nm脂质体与较小的脂组件 (飞艇,) 的共存.
观察到脂质体和较小结构之间的平衡,与VFD中的流体流动模式相关.

结论:

流体装置提供了一种高产量,高通量方法,用于可扩展的脂质体生产.
~110nm脂质体在这些条件下代表了脂体中最稳定的自我组装结构.
该VFD是一个多功能平台,用于脂质体的形成,修饰和机理学研究.

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