在小鼠伤口感染模型中,抗微生物联菌体模仿纳米颗粒对Streptococcus pyogenes具有强大的杀菌作用
Johanna Olesk1, Deborah Donahue2, Jessica Ross3
1Department of Aerospace and Mechanical Engineering, University of Notre Dame Notre Dame Indiana USA pnallath@nd.edu +1 574 631 7868.
Nanoscale advances
|February 15, 2024
概括
抗生素耐药性是一个日益增长的威胁. 研究人员开发了菌体模仿纳米颗粒 (PhaNPs) 与抗菌,有效杀死抗生素耐药细菌,如Streptococcus pyogenes,细胞毒性最小,没有观察到耐药性发展.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 传染性疾病 传染性疾病
背景情况:
- 细菌的抗生素耐药性,特别是*Streptococcus pyogenes*,对公众健康构成重大威胁,导致治疗失败和医院感染.
- 多抗药性细菌的兴起需要开发替代抗菌策略,超越传统的抗生素.
- 纳米材料为创造新型抗菌剂提供了一个有希望的途径.
研究的目的:
- 开发一种无抗生素的纳米粒子系统,模仿菌体,对抗抗生素耐药细菌.
- 合成和描述与合成抗微生物 (PhaNP@Syn71) 结合的模仿菌体的纳米粒子.
- 评估开发的纳米粒子系统的 *in vitro* 和 *in vivo* 疗效和生物相容性.
主要方法:
- 合成核心外纳米粒子 (芯,银涂金纳米球),旨在模仿菌体结构.
- 在纳米粒子表面上对合成抗微生物 (Syn-71) 的结合,形成PhaNP@Syn71.1.
- 在体外评估抗菌活性对*Streptococcus pyogenes*和细胞相容性测试在人类角质细胞.
- 在小鼠伤口感染模型中对PhaNP@Syn71的体内评估.
主要成果:
- PhaNP@Syn71证明了剂量依赖的细菌生长的完全抑制 (>99.99%) *在体外*.
- 这些纳米颗粒对人类皮肤细胞具有极小的细胞毒性,与载体对照剂相当,表明良好的生物相容性.
- 在长时间暴露于PhanP@Syn71.71的低于最低的抑制度后,没有显著的细菌耐药性发展.
- 在体内研究表明,在小鼠模型中,伤口立即稳定,没有感染的愈合,以及高生物相容性.
结论:
- 抗微生物联菌体模仿纳米颗粒 (PhaNP@Syn71) 是一种强大且生物相容的抗菌系统.
- 这种新的系统有效地对抗抗生素耐药的细菌感染,同时规避耐药性的发展.
- 作为对抗具有挑战性的细菌感染的替代治疗策略,PhaNP@Syn71具有显著的潜力.
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