非结核菌:来自官方没有结核病的牛群的皮肤试验牛反应器中发生
Alberto Gomez-Buendia1,2, Julio Alvarez1,2, Javier Bezos1,2
1VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, Spain.
Frontiers in veterinary science
|February 15, 2024
概括
非结核菌 (NTM) 可以在牛结核 (bTB) 检测中引起假阳性. 这项研究在西班牙牛中发现了32种NTM物种,其中Mycobacterium avium复合体成员是最常见的,有助于bTB诊断的准确性.
科学领域:
- 兽医微生物学 兽医微生物学
- 传染性疾病 传染性疾病
- 牛结核病诊断方法 牛结核病诊断方法
背景情况:
- 已知非结核菌 (NTM) 在牛结核 (bTB) 标准皮内结核素试验中会引起非特异性反应.
- 准确的bTB诊断对于根除运动至关重要,NTM的干扰可能会危及群体健康管理和经济稳定.
研究的目的:
- 为了确定在西班牙的牛结核病检测中对诊断干扰负责的NTM的特定物种.
- 来自牛的NTM分离物的多样性,这些牛的bTB检测呈阳性,但来自官方无结核病的群体.
主要方法:
- 在西班牙的bTB根除运动期间,从官方无结核病的牛群中采集了皮肤测试阳性牛的分离物.
- 用PCR和Sanger测序对16SrDNA,hsp65和rpoB基因进行了物种级别的鉴定.
- 基因型鉴定用于分析混合感染,并识别潜在的新型菌根菌种.
主要成果:
- 在308个可靠识别的分离物中,共发现了32种不同的真菌细菌物种.
- 有大量的 (46.1%) Mycobacterium avium复合体 (MAC) 成员,其中包括M. avium亚种. 同类问题是最常见的 (69.0%的MAC).
- 在非MAC成员中 (53.9%),M.nonchromogenicum (27.6%) 和M.bourgelatii (5.6%) 主要存在. 在53个孤立体中检测到混合感染.
结论:
- 这项研究首次描述了NTM物种多样性,这可能会干扰西班牙的bTB诊断.
- 了解特定的NTM物种的流行程度对于提高bTB诊断测试的特异性和加强根除努力至关重要.
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