相关实验视频

Updated: Jul 3, 2025

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

在内皮细胞的HMEC-1细胞中,在登革热病毒感染期间,系统地对来自ncRNAs的小RNA进行计算狩猎

Aimer Gutierrez-Diaz¹, Steve Hoffmann², Juan Carlos Gallego-Gómez³

¹Grupo Rnomica Teórica y Computacional, Departamento de Biología, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia.

Frontiers in bioinformatics

|February 15, 2024

概括

科学家们开发了一种新方法来检测差异表达的小RNA片段 (sfd-RNAs) 和微RNA (miRNAs),这对于理解基因调节和病毒感染至关重要.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

来自非编码RNA (sfd-RNA) 的小RNA片段类似于微RNA (miRNA),使基因调节研究复杂化.
由于注释,读取映射和多重复制问题,现有的miRNA检测方法对于sfd-RNA是不够的.

研究的目的:

开发和验证一种检测差异表达sfd-RNA和正规miRNA的方法.
为了应对小RNA注释,多映射阅读和多复制ncRNAs方面的挑战.

主要方法:

实现了对副本数进行校正的ncRNA注释,用于综合分析.
利用了十六种计算策略,结合了四种对齐器和四种规范化方法.
应用了这种方法来分析感染登革热病毒的人类细胞中的小RNA.

主要成果:

在感染登革热病毒的细胞中成功检测出差异表达的miRNA和sfd-RNA.
确定了对hsa-mir-103a和hsa-mir-494的潜在分子标 (CDK5,PI3/AKT),这些标与DENV感染有关.
提供了一个全面的注释和微分表达式分析框架.

结论:

开发的方法允许精确检测差异表达的sfd-RNA和miRNA.
这种方法对于研究病毒感染中的小RNA作用非常有价值.
对于已识别的分子标,需要进一步的生物验证.

关键词:

登革热病毒感染者不同的表达方式,不同的表达方式.多重映射问题多重映射问题这种ncRNAsRNAs.小小的RNASeq 由tRNA衍生的片段.

更多相关视频

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...