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MORF9依赖的特定塑性RNA编辑抑制了Arabidopsis在糖饥饿下根的生长

Yakun Xie¹, Jinfa Yu¹, Faan Tian¹

¹Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

Plant, cell & environment

|February 15, 2024

概括

丧失MORF9功能会通过破坏塑性RNA编辑来损害植物的发育,影响光合作用和根生长,特别是在缺乏糖的情况下. 糖分水平影响MORF9表达和RNA编辑效率.

科学领域:

植物分子生物学植物分子生物学
RNA编辑机制的编辑机制
植物的发展和应激反应

背景情况:

多个细胞核RNA编辑因子 (MORF) 复合体对于植物细胞核中的C-to-URNA编辑至关重要.
在植物发育和环境反应中,MORF9依赖的塑RNA编辑的确切作用尚未完全被理解.

研究的目的:

调查依赖MORF9的塑性RNA编辑影响植物发育和对环境变化的反应,特别是糖的可用性的机制.

主要方法:

在不同的糖条件下对Morf9突变物的分析.
评估光合作用效率 (PSII),NDH活性和碳水化合物生产.
核基因的基因表达分析,包括糖运输体和糖/能量响应基因.
在野生类型,morf9,gin2/morf9和snrk1/morf9突变物中对特定部位 (matK-706,accD-794,ndhD-383,ndhF-290) 的RNA编辑效率分析.
调查HXK1和SNRK1过度表达对Morf9突变体RNA编辑的影响.

主要成果:

失去MORF9功能导致PSII效率,NDH活性和碳水化合物生产受损.
核基因表达,包括糖运输体,在糖饥饿下迅速升级,在morf9突变者中.
在糖饥饿下,morf9突变体的根生长减弱.
糖的补充影响了MORF9 / MORF2表达和RNA编辑效率在多个网站的MORF9突变.
在双重突变 (gin2/morf9和snrk1/morf9) 中,特定部位的RNA编辑效率进一步下降.
过度表达HXK1和SNRK1在morf9突变体中部分或特异地恢复了RNA编辑效率.

结论:

在植物中维护光合作用效率,碳水化合物代谢和正常根部发育方面,MORF9依赖的塑性RNA编辑是必不可少的.
糖的可用性及其代谢中间体显著影响MORF9依赖的RNA编辑过程.
HXK1和SnRK1信号通路与MORF9相互作用,调节特定的RNA编辑部位,影响植物对营养状况的反应.

关键词:

这是一个MORF字体.根部的发展根部的发展关于糖的反应/监管

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