相关实验视频

Updated: Jul 3, 2025

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Published on: June 19, 2018

人类诱导多能干细胞向心细胞的定向分化

Ziwen Cai^1,2, Miaomiao Zhu^2,3, Li Xu¹

¹Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China (Z.C., L.X., Y.X., W.Y.Y., H.C., R.G., X.Q, J.S., W.Q., N.D.).

|February 15, 2024

概括

研究人员开发了一种有效的协议,以产生功能性人类诱导多能干细胞 (hiPSCs) 衍生的心脏细胞. 这一突破解决了膜性心脏病研究中的一个关键挑战,

科学领域:

干细胞生物学
心血管研究
复原医学

背景情况:

由于缺乏高质量的功能性心脏细胞,因此阻碍了心脏病的研究.
人类诱导的多能干细胞 (hiPSCs) 提供了一个潜在的解决方案,但缺乏确定的分化方案.
控制hiPSC分化为心细胞的分子网络尚不清楚.

研究的目的:

开发一个高效的分化hiPSCs到功能性心脏细胞的协议.
描述这些衍生细胞的转录和功能性质.
阐明涉及的差异化轨迹和监管网络.

主要方法:

在hiPSC中连续激活Wnt,BMP4,VEGF和NFATc1信号通路.
使用基因表达特征和功能测试对hiPSC衍生的细胞进行表征.
纵向单细胞RNA测序以绘制差异化途径并确定关键调节者.

主要成果:

一个高效的方案产生了hiPSC衍生的内皮样细胞 (CD144+) 和间皮样细胞 (CD144-).
这些细胞在基因表达和功能上与原发性心细胞非常相似 (例如,管道形成,原分泌).
单细胞RNA测序确定了关键基因 (NOTCH1,HEY1,MEF2C),途径 (TGF-β,Wnt,NOTCH) 和转录因子介导分化,揭示了与胚胎发育相似的轨迹.

结论:

这项研究提出了第一个有效的策略,用于产生功能性hiPSC衍生内皮样和间皮样细胞.
这项研究阐明了hiPSCs的分化轨迹和转录动力学.
这些发现为推进膜心脏病研究和治疗开发提供了宝贵的资源.

关键词:

细胞分化内皮细胞心脏门诱导多能干细胞单细胞基因表达分析

更多相关视频

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

相关概念视频

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...