在Leptosphaeria maculans中作为协同波的效应基因表达的调节:一个两人游戏
Colin Clairet1, Elise J Gay1, Antoine Porquier1
1Université Paris-Saclay, INRAE, UR BIOGER, 91120, Palaiseau, France.
The New phytologist
|February 15, 2024
概括
植物病原菌在效应基因表达上使用双重控制. 一个转录因子 (LmPf2) 和KMT1介导的组素修饰 (H3K9me3) 共同调节真菌病原性和效应基因.
科学领域:
- 植物病理学 植物病理学
- 分子菌学 分子菌学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 效应基因对于植物病原菌来说至关重要,但它们的调节仍然不太了解.
- 染色体重塑,特别是KMT1沉积的H3K9me3基因组修饰,涉及到像*Leptosphaeria maculans*这样的真菌中的效应器基因调节.
- 有证据表明,对于效应基因控制来说,需要一个二级调节层,可能涉及转录因子.
研究的目的:
- 调查转录因子LmPf2的作用及其与KMT1的相互作用,以调节*L. maculans*中的*效应因子基因表达和病原性.
- 阐明控制植物病原性真菌中效应体基因表达的双重控制机制.
主要方法:
- 对Pf2 (LmPf2),一种Zn2Cys6真菌特异性转录因子的*L. maculans*正基因的识别和表征.
- 在野生类型和L. maculans*的突变菌株中,LmPf2*的失活和过度表达.
- 变种物的功能分析,以评估病原性和效应基因表达模式.
主要成果:
- 在L. maculans*中,LmPf2在建立病变发生过程中起着至关重要的作用.
- LmPf2显著影响诱导效应基因表达,特别是当KMT1-介导的抑制得到缓解时.
- 这项研究表明,一种新的双重控制机制用于植物病原性真菌中的效应器基因表达.
结论:
- 在*L. maculans*中,效应基因表达处于双重调控之下,涉及表观遗传修饰 (KMT1/H3K9me3) 和特定的转录因子 (LmPf2).
- 这种双重控制对于真菌致病性和在宿主植物 (油菜) 中有效建立疾病至关重要.
- 这些发现提供了结合转录和表观遗传调节系统的第一个证据,该系统控制了植物病原体中的效应基因.
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