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在芯片上设计瘤免疫微环境,研究T细胞与巨细胞在乳腺癌进展中的相互作用

Twinkle Jina Minette Manoharan¹, Kalpana Ravi¹, Abhirami P Suresh^2,3

¹School of Biological and Health Systems Engineering (SBHSE), Arizona State University, Tempe, AZ, 85287, USA.

Advanced healthcare materials

|February 15, 2024

概括

巨细胞显著推动乳腺癌的进展,而T细胞减缓了它. 这项研究使用了3D瘤芯片模型来探索瘤免疫微环境 (TIME) 内的免疫细胞相互作用.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
生物技术是生物技术.

背景情况:

瘤免疫微环境 (TIME) 对乳腺癌的进展和治疗反应至关重要.
针对TIME内的免疫细胞是新型癌症治疗的关键策略.
了解免疫细胞交叉通话对于克服瘤免疫逃逸至关重要.

研究的目的:

为了研究巨细胞和T细胞在乳房内的交叉声. 时间.
阐明这些免疫细胞在推动瘤进展中的作用.
为了利用一个3D有机型瘤芯片 (TOC) 模型来研究时间动态.

主要方法:

开发一个三培养3D有机型瘤芯片 (TOC) 模型.
在3D矩阵中嵌入巨细胞和T细胞,并与瘤和树皮细胞一起.
细胞入侵,形态测量,流细胞测量和细胞因子表达 (勒,RANTES) 的分析.

主要成果:

巨细胞对瘤进展有显著的贡献.
T细胞与癌细胞和巨细胞的迁移减少有关.
在三种培养系统中观察到素和RANTES (受激活调节,正常的T细胞表达和分泌) 的升高.

结论:

免疫细胞群,特别是巨细胞和T细胞,在乳腺癌的进展中发挥着关键作用.
时间内的复杂交叉声会显著影响瘤的行为.
3D TOC模型为剖析 TIME 相互作用提供了一个有价值的平台.

关键词:

一个T细胞细胞.癌症免疫免疫癌症巨细胞是什么?巨细胞是什么?微流体学在微流体学方面组织类型的器官类型.芯片上的瘤 (TOC)瘤免疫微环境 (TIME)

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