在Cryptochrome信号传输中,一个PDZ支架/CaM介导的通路
Massimo Bellanda1, Milena Damulewicz2, Barbara Zambelli3
1Department of Chemical Sciences, University of Padova, Padova, Italy.
Protein science : a publication of the Protein Society
|February 15, 2024
概括
离子 (Ca2+) 和卡尔莫杜林 (CaM) 调节密码色素,对昼夜节律至关重要. 这项研究揭示了一种涉及人类CRY2和MUPP1的Ca2+/CaM介导的新型信号通路,该通路可能在各个物种中得到保护.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 加密染色体是昼夜时钟的关键组成部分,调节日常生理节奏.
- 新出现的证据表明,加密染色体参与非昼夜轨道,表明复杂的调节.
- 精确的调节加密色素功能的分子机制尚未完全理解.
研究的目的:
- 研究新型蛋白质相互作用和影响加密色素功能的信号通路.
- 阐明离子和卡尔莫杜林在哺乳动物密码染色体调节中的作用.
- 探索介导的加密色调节的进化保存.
主要方法:
- 同免疫沉测试检测蛋白质复合体的形成.
- 定位实验,以评估依赖的相互作用.
- 西方涂抹和其他生化技术来分析蛋白质相互作用.
主要成果:
- 人类CRY2 (hCRY2) 与脚手架蛋白质多PDZ域蛋白1 (MUPP1) 形成了一个稳定的复合体.
- 这种相互作用依赖于结合蛋白卡尔莫杜林 (CaM),需要离子 (Ca2+).
- 发现了一种新的/卡尔莫杜林介导的哺乳动物密码染色体的调节机制.
结论:
- 离子和卡尔莫杜林介导哺乳动物密码染色体的新型调节途径,涉及hCRY2和MUPP1.
- 这种依赖Ca2+/CaM的信号通路代表了从Drosophila到哺乳动物的潜在的进化保存机制.
- 了解这些相互作用增强了我们在更广泛的昼夜时钟网络中的加密色素功能知识.
相关概念视频
Assembly of Signaling Complexes
5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K
MAPK Signaling Cascades
5.5K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.5K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Biological Clocks and Seasonal Responses
34.6K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.6K
Intracellular Signaling Cascades
46.9K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
46.9K


