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通过单个活细胞中的Au纳米粒子穿过细胞膜的道电子转移

Rujia Liu1, Dengchao Wang1

  • 1School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P. R. China.

Nano letters
|February 15, 2024
PubMed
概括

我们开发了一种简单的电化学方法,使用电子道来检测单个活细胞内的反应性氧物种 (ROS). 这种技术在没有侵入性纳米电极透的情况下量化细胞内ROS水平,为细胞分析提供了潜力.

关键词:
在AU纳米粒子.碰撞电化学 碰撞电化学一个单细胞的生命细胞.道化电子转移的电子转移

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Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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