相关实验视频
Updated: Jul 3, 2025

08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
7.9K
通过将其分成不同的内分体域来激活Notch的替代机制
Hideyuki Shimizu1, Samira Hosseini-Alghaderi1, Simon A Woodcock1
1School of Biological Sciences, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK.
The Journal of cell biology
|February 15, 2024
概括
划分受体的激活是由其位于内分体微域的位置控制的. 德尔特克斯和ESCRT复合体调节了这些域之间的Notch运动,影响了不同的激活通路.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 膜微域创建专门的环境,影响信号接收器的功能.
- 诺奇信号通路对于细胞间的通信和发育至关重要.
研究的目的:
- 为了研究内体微域在调节Drosophila中Notch受体激活中的作用.
- 阐明微域本地化影响Notch激活通路的机制.
主要方法:
- 在Drosophila内分体上识别和表征不同的微域 (脂质和克拉/ESCRT-0).
- 对Notch受体局部化和激活的分析,以应对Deltex和ESCRT复合物的遗传干扰.
- 研究ADAM10和TRPML通道在微域特异性Notch激活中的作用.
主要成果:
- 口激活发生在脂质飞艇和克拉/ESCRT-0微域内通过不同的连接体独立机制.
- 德尔特克斯 (Deltex) 基因酶调节了微域之间的切口转移,影响了通过TRPML通道的激活.
- ESCRT复合体作为守门员,扰动导致改变的Notch激活机制 (ADAM10依赖或独立).
结论:
- 内体微域的组成决定了Notch激活机制.
- 诺奇调节器,内体贩运机械和诺奇基因之间的相互作用决定了其局部化和激活途径.
- 这项研究揭示了一种新的Notch信号调节层,该调节层位于内体膜组织层.
相关概念视频
Notch Signaling Pathway
4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
The Early Endosome: Endocytosis of Transferrin
3.3K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.3K
Tail-anchoring of Proteins in the ER Membrane
3.1K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.1K
Pinching-off of Coated Vesicles
3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K

